apc anti mouse cd4 (Elabscience Biotechnology)

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Elabscience Biotechnology apc anti mouse cd4

Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and <t>CD4</t> surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Apc Anti Mouse Cd4, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc+anti+mouse+cd4/APC+Anti-Mouse+CD4+Antibody/pmc13091351-201-21-35
Average 94 stars, based on 36 article reviews

apc anti mouse cd4 - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment"

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

Journal: Molecular Therapy Oncology

doi: 10.1016/j.omton.2026.201185

Figure Legend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Techniques Used: Flow Cytometry

Incubation:

Article Title: Effect and mechanisms of shikonin on breast cancer cells in vitro and in vivo
Article Snippet: One portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11] (Elabscience, China), Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2] (Elabscience), APC Anti-Mouse CD4 Antibody[GK1.5] (Elabscience, China) and fluorescein isothiocyanate (FITC) Anti-Mouse CD8a Antibody[53 − 6.7] (Elabscience) diluted in cell staining buffer (Elabscience, China) at 4 °C in the dark for 30 min. .. The other portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11], Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2], APC Anti-Mouse CD4 Antibody[GK1.5], and PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] (Elabscience, China) diluted in cell staining buffer at 4 °C in the dark for 30 min. .. Fixed membrane breaking and nucleation breaking solutions (Biolegend, USA) were used to disrupt cell membranes and nuclei, respectively, according to the specifications to facilitate staining with phycoerythrin (PE) Anti-Mouse Foxp3 Antibody [3G3] (Elabscience, China).

Article Title: Effect and mechanisms of shikonin on breast cancer cells in vitro and in vivo
Article Snippet: .. One portion was incubated with Elab Fluor® Violet 450 Anti-Mouse CD45 Antibody[30F11] (Elabscience, China), Elab Fluor® Red 780 AntiMouse CD3 Antibody[17A2] (Elabscience), APC Anti-Mouse CD4 Antibody[GK1.5] (Elabscience, China) and fluorescein isothiocyanate (FITC) Anti-Mouse CD8a Antibody[53 − 6.7] (Elabscience) diluted in cell staining buffer (Elabscience, China) at 4 °C in the dark for 30 min. .. The other portion was incubated with Elab Fluor® Violet 450 Anti-Mouse CD45 Antibody[30-F11], Elab Fluor® Red 780 Anti-Mouse CD3 Antibody[17A2], APC AntiMouse CD4 Antibody[GK1.5], and PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] (Elabscience, China) diluted in cell staining buffer at 4 °C in the dark for 30 min.

Article Title: Effect and mechanisms of shikonin on breast cancer cells in vitro and in vivo
Article Snippet: .. One portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11] (Elabscience, China), Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2] (Elabscience), APC Anti-Mouse CD4 Antibody[GK1.5] (Elabscience, China) and fluorescein isothiocyanate (FITC) Anti-Mouse CD8a Antibody[53 − 6.7] (Elabscience) diluted in cell staining buffer (Elabscience, China) at 4 °C in the dark for 30 min. .. The other portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11], Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2], APC Anti-Mouse CD4 Antibody[GK1.5], and PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] (Elabscience, China) diluted in cell staining buffer at 4 °C in the dark for 30 min.

Article Title: Qing’e Pills Ameliorates Osteoporosis by Regulating Gut Microbiota and Th17/Treg Balance in Ovariectomized Rats
Article Snippet: Subsequently, the tissue suspension was incubated with a protein transport inhibitor enzyme (S1753, Beyotime, Shanghai, China) at a concentration of 3 μM. .. Subsequently, APC-anti-mouse-CD4 (213644, Elabscience, Houston, TX, USA), PE-cy5-anti-mouse-CD4 (15–0041-81, eBioscience, San Diego, CA, USA), and APC-anti-mouse-CD25 antibodies (208614, Elabscience) were added to the suspension, and the mixture was incubated in the dark at 37°C for 30 min. .. Subsequently, a fixative solution (2330512, Invitrogen, Waltham, MA, USA) was added, and the mixture was incubated for another 30 min.

Article Title: Qing’e Pills Ameliorates Osteoporosis by Regulating Gut Microbiota and Th17/Treg Balance in Ovariectomized Rats
Article Snippet: Subsequently, the tissue suspension was incubated with a protein transport inhibitor enzyme (S1753, Beyotime, Shanghai, China) at a concentration of 3 μM. .. Subsequently, APC-anti-mouse-CD4 (213644, Elabscience, Houston, TX, USA), PE-cy5-antimouse-CD4 (15–0041-81, eBioscience, San Diego, CA, USA), and APC-anti-mouse-CD25 antibodies (208614, Elabscience) were added to the suspension, and the mixture was incubated in the dark at 37°C for 30 min. .. Subsequently, a fixative solution (2330512, Invitrogen, Waltham, MA, USA) was added, and the mixture was incubated for another 30 min.

Staining:

Article Title: Effect and mechanisms of shikonin on breast cancer cells in vitro and in vivo
Article Snippet: .. One portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11] (Elabscience, China), Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2] (Elabscience), APC Anti-Mouse CD4 Antibody[GK1.5] (Elabscience, China) and fluorescein isothiocyanate (FITC) Anti-Mouse CD8a Antibody[53 − 6.7] (Elabscience) diluted in cell staining buffer (Elabscience, China) at 4 °C in the dark for 30 min. .. The other portion was incubated with Elab Fluor ® Violet 450 Anti-Mouse CD45 Antibody[30-F11], Elab Fluor ® Red 780 Anti-Mouse CD3 Antibody[17A2], APC Anti-Mouse CD4 Antibody[GK1.5], and PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] (Elabscience, China) diluted in cell staining buffer at 4 °C in the dark for 30 min.

CCK-8 Assay:

Article Title: A smart all-in-one strategy based on hybrid bacteria with targeting drug delivery and spatiotemporal drug release to boost the synergistic therapeutic efficacy against TNBC
Article Snippet: Mouse IL-6, IFN-γ and TNF-α and HMGB1 enzyme-linked immunosorbent assay (ELISA) kits were provided by Enzyme-linked Biotechnology Co., Ltd (Shanghai, China). .. Cell counting kit-8 (CCK-8), PE Anti-mouse CD3, FITC Anti-mouse CD8a and APC Anti-mouse CD4 were obtained from Elabscience Biotechnology Co., Ltd (Wuhan, China). .. His-tag XP Rabbit mAb was purchased from Cell Signaling Technology (Danvers, USA).

Suspension:

Article Title: Qing’e Pills Ameliorates Osteoporosis by Regulating Gut Microbiota and Th17/Treg Balance in Ovariectomized Rats
Article Snippet: Subsequently, the tissue suspension was incubated with a protein transport inhibitor enzyme (S1753, Beyotime, Shanghai, China) at a concentration of 3 μM. .. Subsequently, APC-anti-mouse-CD4 (213644, Elabscience, Houston, TX, USA), PE-cy5-antimouse-CD4 (15–0041-81, eBioscience, San Diego, CA, USA), and APC-anti-mouse-CD25 antibodies (208614, Elabscience) were added to the suspension, and the mixture was incubated in the dark at 37°C for 30 min. .. Subsequently, a fixative solution (2330512, Invitrogen, Waltham, MA, USA) was added, and the mixture was incubated for another 30 min.

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